DNA pol ι rabbit polyclonal antibody
应用:Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
产品名:DNA pol ι rabbit Polyclonal Antibody
货号:KLP28216
种类(Category)
Primary antibodies
宿主(Host)
Rabbit
反应种属(Species specificity)
Human
应用实验(Tested applications)
WB,ELISA
克隆性(Clonality)
Polyclonal
偶连物(Conjugation)
Unconjugated
免疫原(Immunogen)
The antiserum was produced against synthesized peptide derived from human POLI. AA range:641-690
状态(Form)
Liquid
存放条件(Storage)
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 °C for frequent use. Store at -20 to -80 °C for twelve months from the date of receipt.
纯化方式(Purity)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
产品背景:
catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,cofactor:Magnesium.,domain:The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.,function:Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.,similarity:Belongs to the DNA polymerase type-Y family.,similarity:Contains 1 umuC domain.,subcellular location:Accumulates at replication forks after DNA damage.,subunit:Binds REV1L (By similarity). Binds POLH.,tissue specificity:Ubiquitous. Highly expressed in testis.,
特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!