胱天蛋白酶7(CASP7)重组蛋白
  • 胱天蛋白酶7(CASP7)重组蛋白

胱天蛋白酶7(CASP7)重组蛋白

Recombinant Caspase 7 (CASP7)
胱天蛋白酶7(CASP7)重组蛋白应用:SDS-PAGE; WB。
价格 3680
规格: 50µg/200µg/500µg/1mg/5mg
货号: IC849Ra

胱天蛋白酶7(CASP7)重组蛋白

Recombinant Caspase 7 (CASP7)

CMH1; ICE-LAP3; MCH3; Caspase 7 Apoptosis-Related Cysteine Peptidase; Cysteine Aspartate Protease 7; Cysteinyl Aspartate Specific Proteinases 7

[ PROPERTIES ]

Residues: Met1~Asn250

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: F7F246

Host: E. coli

Subcellular Location: . Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 5.2

Predicted Molecular Mass: 31.6kDa

Accurate Molecular Mass: 32kDa as determined by SDS-PAGE reducing conditions.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to

give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

MTDDQDCAAE LEMADSSTED GVDAKPDRST IISSLLWKKK KNASMCPVST TRDRVPTYLYRMDFEKMGKC IIINNKNFDK ATGMDVRNGT DKDAEALFKC FRSLGFEVTV YNDCSCAKMQDLLRRASEED HSNSACFACV LLSHGEENLI YGKDGVTPIK DLTAHFRGDR CKTLLEKPKLFFIQACRGTE LDDGIQADSG PINDTDANPR YKIPVEADFL FAYSTVPGXX SWRNPGKGSWFVQALCSILN

特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途! 

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