谷光甘肽合成酶(GSS)重组蛋白

谷光甘肽合成酶(GSS)重组蛋白

Recombinant Glutathione Synthetase (GSS)

Glutathione synthase

[ PROPERTIES ]

Residues: Tyr270~Val474

Tags: N-terminal His-Tag

Accession: P46413

Host: E. coli

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 5.9

Predicted Molecular Mass: 23.8kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

Y MPSQY NA QNW EA R L L LE RS C AAK CP DIATQ LAG TKKVQ QE LS RVG L LEA LLPGQPEAVAR LRATFAGLYS LDMGEEGDQA VAEALAAPSH FVLKPQREGG GNNFYGEEMVHALEQLKDSE ERASYILMEK IEPEPFRNCL LRPGSPAQVV QCISELGIFG VYVRQGTTLVMNKHVGHLLR TKAIEHADGG VAAGVAVLDN PYPV

[ REFERENCES ]

1. Huang C.-S., et al. (1995) Proc. Natl. Acad. Sci. U.S.A. 92:1232-1236.

2. Luo J.L., et al. (2000) Biochem. Biophys. Res. Commun. 275:577-581.

3. Yang H., et al. (2002) J. Biol. Chem. 277:35232-35239.

4. Yang H., et al. (2005) Biochem. J. 391:399-408.

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