葡萄糖激酶调节蛋白(GKRP)重组蛋白

葡萄糖激酶调节蛋白(GKRP)重组蛋白

Recombinant Glucokinase Regulatory Protein (GKRP)

GCKR; Glucokinase(hexokinase 4)regulator

[ PROPERTIES ]

Residues: Gln91~Ala286 (Accession # Q14397),

with N-terminal His-Tag.

Host: E. coli

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 8.6

Predicted Molecular Mass: 22.3kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGSEF-QEVLKEPDGG LVVLSGGGTS GRMAFLMSVS FNQLMKGLGQKPLYTYLIAG GDRSVVASRE GTEDSALHGI EELKKVAAGK KRVIVIGISV GLSAPFVAGQMDCCMNNTAV FLPVLVGFNP VSMARNDPIE DWSSTFRQVA ERMQKMQEKQ KAFVLNPAIGPEGLSGSSRM KGGSATKILL ETLLLAAHKT VDQGIA

[ REFERENCES ]

1. Warner J.P., et al. (1995) Mamm. Genome 6:532-536. 

2. Hayward B.E., et al. (1998) Genomics 49:137-142. 

3. de la Iglesia N., et al. (1999) FEBS Lett. 456:332-338. 

4. Vaxillaire M., et al. (2008) Diabetes 57:2253-2257.

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