层粘连蛋白γ3(LAMC3)重组蛋白
Recombinant Laminin Gamma 3 (LAMC3)
LAM-C3
[ PROPERTIES ]
Residues: Cys901~Gly1100
Tags: Two N-terminal Tags, His-tag and GST-tag
Accession: Q9Y6N6
Host: E. coli
Subcellular Location: Secreted, extracellular
space, extracellular matrix, basement membrane.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 7.8
Predicted Molecular Mass: 51.7kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
CYPGFF DLQP GRGCRSCK CH PLGSQEDQCH PKTGQCTCRP GVTGQA CDRCQL GF FG FSIK GC RA C RCS P L GA AS AQ C HE N GT CV CRP GF E GY K C D RC H DNFFLTADGTHC QQCPSCYALV KEEAAKLKAR LTLTEGWLQG SDCGSPWGPL DILLGEAPRGDVYQGHHLLP GAREAFLEQM MSLEGAVKAA REQLQRLNKG
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