胸腺嘧啶核苷磷酸化酶(TP)重组蛋白

胸腺嘧啶核苷磷酸化酶(TP)重组蛋白

Recombinant Thymidine Phosphorylase (TP)

TYMP; PDECGF; ECGF1; MNGIE; TP; HPD-ECGF; TdRPase; Platelet-Derived Endothelial Cell Growth Factor; Gliostatin

[ PROPERTIES ]

Residues: Ile33~Ala290

Tags: Two N-terminal Tags, His-tag and GST-tag

Accession: Q5FVR2

Host: E. coli

Purity: >90%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 5.8

Predicted Molecular Mass: 59.2kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

IRLKRNGG HLSEADIRNF VHALMDGRAQ DTQIGAMLMA IRLQGMDLEE TSVLTQALAESG Q Q LEWP KA WH QQ LV DK HS TG GVG DK VS L VLA PA LAA C G CK VPMIS G RSLGHTGGTLDK LESIPGFSVT QSPEQMLQIL EEVGCCIVGQ SEKLVPADGI LYAARDVTATVDSVPLITAS ILSKKAVEGL STLVVDVKFG GAAVFPDQEK ARELAKMLVR VGMGLGLQVAAALTAMDNPL GRNVGHTLEV EEALLCLDGA

[ REFERENCES ]

1. Kubiak R., et al. (1999) Z. Naturforsch., C, J. Biosci. 54:1096-1102. 

2. Zhao B., et al. (2000) Jpn. J. Cancer Res. 91:331-336. 

3. Yanagi Y., et al. (2003) Invest. Ophthalmol. Vis. Sci. 44:751-754. 

4. Miszczak-Zaborska E., et al. (2004) Gynecol. Oncol. 94:86-92.

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