甘露糖受体C1(MRC1)重组蛋白

甘露糖受体C1(MRC1)重组蛋白

Recombinant Mannose Receptor C Type 1 (MRC1)

CD206; CLEC13D, CLEC13DL, MRC1L1; C-type lectin domain family 13 member D-like; Macrophage mannose receptor 1-like protein 1

[ PROPERTIES ]

Residues: Leu225~Ser492 (Accession # Q61830),

with two N-terminal Tags, His-tag and T7-tag. Host: E. coli

Subcellular Location: Endosome membrane;

Single-pass type I membrane protein. Cell

membrane.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 6.9

Predicted Molecular Mass: 34.1kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF - LTGILY QINSKSALTWHQARASCKQQ NADLLSVTEI HEQMYLTGLT SSLSSGLWIG LNSLSVRSGW QWAGGSPFRYLNWLPGSPSS EPGKSCVSLN PGKNAKWENL ECVQKLGYIC KKGNNTLNPF IIPSASDVPTGCPNQWWPYA GHCYRIHREE KKIQKYALQA CRKEGGDLAS IHSIEEFDFI FSQLGYEPNDE LWI G L N DI K IQ M Y F EW S D G TP V T F T KW L P GE P S HE N N R Q E D C V VM K G K DGYWADRACEQ PLGYICKMVS QS

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