蛋白磷酸酶2A激活因子调节因子亚基4(PPP2R4)重组蛋白

蛋白磷酸酶2A激活因子调节因子亚基4(PPP2R4)重组蛋白

Recombinant Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4)

PP2A; PR53; PTPA; Phosphotyrosyl Phosphatase Activator; PP2A Phosphatase Activator; PP2A, subunit B', PR53 isoform; Serine/threonine-protein phosphatase 2A activator

[ PROPERTIES ]

Residues: Met1~Gly323

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: B2RYQ2

Host: E. coli

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 5.9

Predicted Molecular Mass: 40.3kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

MAEGERQPPP DSSEETPSAT PTFIIPKKEI HTVPDMGKWK RSQAYADYIG FILTLNEGVKGKKLTFDYKV SEAIEKLVAL LDTLDRWIDE TPPVDQPSRF GNKAYRTWYA KLDQEAENLVATVIPTNLAA AVPEVAVYLK EAVGNSTRID YGTGHEAAFA AFLCCLCKIG VLRVDDQVAIVFKVFDRYLE VMRKLQKTYR MEPAGSQGVW GLDDFQFLPF IWGSSQLIDH PHLEPRHFVDEKAVSENHKD YMFLQCILFI TEMKTGPFAE HSNQLWNISA VPSWSKVNQG LIRMYKAECLEKFPVIQHFK FGSLLPIHPV TSG

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