尼克酰胺腺嘌呤二核苷酸磷酸氧化酶4(NOX4)重组蛋白
Recombinant NADPH Oxidase 4 (NOX4)
KOX; KOX-1; RENOX; Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4
[ PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Asp220~Asp392
Tags: N-terminal His-Tag
Tissue Specificity: Kidney, Heart, Liver Cancer. Subcellular Location: Endoplasmic reticulum membrane; Multi-pass membrane
protein. Cell junction, focal adhesion. Nucleus. Purity: >95%
Traits: Freeze-dried powder
Buffer formulation: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01%
sarcosyl and Proclin300. Original Concentration: 200ug/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive
Labeling. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 6.7
Predicted Molecular Mass: 21.2kDa
Accurate Molecular Mass: 21kDa as determined by SDS-PAGE reducing conditions.
[ USAGE ]
Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8
oC for one month. Aliquot and store at -80
oC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37oC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date under
appropriate storage condition.
[ SEQUENCE ]
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