血管生长素(ANG)重组蛋白

血管生长素(ANG)重组蛋白

Recombinant Angiogenin (ANG)

RNASE5; Ribonuclease,RNase A Family 5

[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]

Protein Protein Protein Protein Names: Angiogenin

Gene Names: ANG

Size: 100µg

Source: Recombinant

Expression Expression Expression Expression Host: E.coli

Function: Function: Function: Function: May function as a tRNA-specific ribonuclease that abolishes protein

synthesis by specifically hydrolyzing cellular tRNAs. Binds to actin on the surface of

endothelial cells; once bound, angiogenin is endocytosed and translocated to the

nucleus. Angiogenin induces vascularization of normal and malignant tissues.

Angiogenic activity is regulated by interaction with RNH1 in vivo.

Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Secreted

[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]

Residues Residues Residues Residues: Lys1~Gln123 (Accession # P31346), with a N-terminal His-tag.

Grade & Purity: Purity: Purity: Purity: >97%, 15.58 kDa as determined by SDS-PAGE reducing conditions.

Form & Buffer: Supplied as lyophilized form in PBS, pH 7.4.

Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg(determined by the LAL method). Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA;IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 15.58 kDa

[ PREPARATION PREPARATION PREPARATION PREPARATION ]

Reconstitute in PBS.

[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

Storage: Storage: Storage: Storage: Store at 4oC for short term storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term

storage. Avoid repeated freeze/thaw cycles.

Valid period: period: period: period: 12 months stored at -80oC.

[ BACKGROUND BACKGROUND BACKGROUND BACKGROUND]

The target protein is fused with a His-tag and its sequence is listed below. The first Met is an initiator amino acid. Moreover, Gly and Ser are added to improve the flexibility of N-terminus at both ends of the His-tag, which willincrease the chelating ability of the tag to Ni-Sepharose during purification.

MGHHHHHHSGSEF-KDEDRYTHFL TQHYDAKPKG RDGRYCESIM KQRGLTRPCK EVNTFIHGTRNDIKAICNDK NGEPYNNFRR SKSPFQITTC KHKGGSNRPP CGYRATAGFR TIAVACENGL PVHFDESFII TSQ

[ REFERENCES REFERENCES REFERENCES REFERENCES ]

1. Bond M.D., et al. (1993) Biochim. Biophys. Acta 1162:177-186

2. Kurachi, K., et al. (1985) Biochemistry. 24: 5495-5499.

3. Rybak, S. M., et al. (1987) Biochem. Biophys. Res. Commun. 146: 1240-1248.

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