粘蛋白3(MUC3)重组蛋白

粘蛋白3(MUC3)重组蛋白

Recombinant Mucin 3 (MUC3)

MUC3A; Mucin 3A,Intestinal

[ PROPERTIES ]

Residues: Cys2176~Ala2280

Tags: Two N-terminal Tags, His-tag and GST-tag

Accession: Q02505

Host: E. coli

Subcellular Location: Secreted, Membrane;

Single-pass membrane protein.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 5.4

Predicted Molecular Mass: 43.7kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this prtein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

CDNGG TWEQGQCACL PGFSGDRCQL QTRCQNGGQW DGLKCQCPST FYGSSCEFAVEQVDLDVVET EVGMEVSVDQ QFSPDLNDNT SQAYRDFNKT FWNQMQKIFA

[ REFERENCES ]

1. Hillier L.W., et al. (2003) Nature 424:157-164. 

2. Gum J.R. Jr., et al. (2003) J. Biol. Chem. 278:49600-49609. 

3. Pratt W.S., et al. (2000) Biochem. Biophys. Res. Commun. 275:916-923.

 4. Kyo K., et al. (2001) J. Hum. Genet. 46:5-20.

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