信号调节蛋白γ(SIRPg)重组蛋白

信号调节蛋白γ(SIRPg)重组蛋白

Recombinant Signal Regulatory Protein Gamma (SIRPg)

CD172g; CD172-G; SIRP-G; SIRP-B2; SIRPB2; SIRP-b2; SIRP-beta-2; SIRPgamma; CD172 antigen-like family member B; Signal-regulatory protein beta-2

[ PROPERTIES ]

Residues: Pro111~Ile372

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q9P1W8

Host: E. coli

Subcellular Location: Membrane; Single-pass

type I membrane protein.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in

100mM NaHCO3, 500mM NaCl, pH8.3, containing

1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%

trehalose, and preservative.

Predicted isoelectric point: 6.6

Predicted Molecular Mass: 32.5kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

 Store at 2-8

oC for one month.

 Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

PADVGTYYCV KFRKGSPENV EFKSGPGTEM ALGAKPSAPV VLGPAARTTP EHTVSFTCESHGFSPRDITL KWFKNGNELS DFQTNVDPTG QSVAYSIRST ARVVLDPWDV RSQVICEVAHVTLQGDPLRG TANLSEAIRV PPTLEVTQQP MRVGNQVNVT CQVRKFYPQS LQLTWSENGNVCQRETASTL TENKDGTYNW TSWFLVNISD QRDDVVLTCQ VKHDGQLAVS KRLALEVTVHQKDQSSDATP GPASSLTALL LI

[ REFERENCES ]

1. Ichigotani Y., et al. (2000) J. Hum. Genet. 45:378-382.

2. Piccio L., et al. (2005) Blood 105:2421-2427.

3. Wollscheid B., et al. (2009) Nat. Biotechnol. 27:378-386.

4. Hatherley D., et al. (2008) Mol. Cell 31:266-277.

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