组织因子通道抑制因子(TFPI)重组蛋白

组织因子通道抑制因子(TFPI)重组蛋白

Recombinant Tissue Factor Pathway Inhibitor (TFPI)

TFI; EPI; LACI; TFPI1; Lipoprotein-Associated Coagulation Inhibitor; Extrinsic pathway inhibitor

[ PROPERTIES ]

Residues: Asp29~Glu210 (Accession # P10646),

with N-terminal His-Tag.

Host: E. coli

Subcellular Location: Secreted. Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 5.2

Predicted Molecular Mass: 22.6kDa

Accurate Molecular Mass: 18kDa as determined by SDS-PAGE reducing conditions.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to

give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSG SEF- DS EEDEEHTIIT DTELPPLKLM HSFCAFKADD GPCKAIMKRFFFNIFTRQCE EFIYGGCEGN QNRFESLEEC KKMCTRDNAN RIIKTTLQQE KPDFCFLEEDPGICRGYITR YFYNNQTKQC ERFKYGGCLG NMNNFETLEE CKNICEDGPN GFQVDNYGTQLNAVNNSLTP QSTKVPSLFE

[ REFERENCES ]

1. Wun T.C., et al. (1988) J. Biol. Chem. 263:6001-6004.

 2. Girard T.J., et al. (1989) Thromb. Res. 55:37-50.

 3. Girard T.J., et al. (1991) J. Biol. Chem. 266:5036-5041. 

4. Liu T., et al. (2005) J. Proteome Res. 4:2070-2080.

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