钠钾氯协同转运蛋白2(NKCC2)重组蛋白

钠钾氯协同转运蛋白2(NKCC2)重组蛋白

Recombinant Na-K-Cl Cotransporter 2 (NKCC2)

SLC12A1; NKCC2; Solute Carrier Family 12 Member 1; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; Kidney-specific Na-K-Cl symporter

[ PROPERTIES ]

Residues: Arg814~Ser1099

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q13621

Host: E. coli

Subcellular Location: Membrane; Multi-pass

membrane protein.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 9.0

Predicted Molecular Mass: 36.8kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

RISQGFD ISQVLQVQEE LERLEQERLA LEATIKDNEC EEESGGIRGL FKKAGKLNITKTTPKKDGSI NTSQSMHVGE FNQKLVEAST QFKKKQEKGT IDVWWLFDDG GLTLLIPYILTLRKKWKDCK LRIYVGGKIN RIEEEKIVMA SLLSKFRIKF ADIHIIGDIN IRPNKESWKVFEEMIEPYRL HESCKDLTTA EKLKRETPWK ITDAELEAVK EKSYRQVRLN ELLQEHSRAANLIVLSLPVA RKGSISDLLY MAWLEILTKN LPPVLLVRGN HKNVLTFYS

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