Toll样受体衔接分子2(TICAM2)重组蛋白

Toll样受体衔接分子2(TICAM2)重组蛋白

Recombinant Toll Like Receptor Adaptor Molecule 2 (TICAM2)

TRAM; TIRAP3; TIRP; Toll/Interleukin-1 Receptor Domain-Containing; TIR domain-containing adapter molecule 2; Toll-like receptor adaptor Protein 3; TRIF-related adapter molecule

[ PROPERTIES ]

Residues: Ser22~Gln205 (Accession # Q86XR7),

with two N-terminal Tags, His-tag and T7-tag. Host: E. coli

Subcellular Location: Cytoplasm. Golgi

apparatus. Cell membrane. Endoplasmic

reticulum. Early endosome membrane. Late

endosome membrane.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 5.0

Predicted Molecular Mass: 24.7kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SVDTSPGYH ESDSKKSEDLSLCNVAEHSN TTEGPTGKQE GAQSVEEMFE EEAEEEVFLK FVILHAEDDT DEALRVQNLLQDDFGIKPGI IFAEMPCGRQ HLQNLDDAVN GSAWTILLLT ENFLRDTWCN FQFYTSLMNSVNRQHKYNSV IPMRPLNNPL PRERTPFALQ TINALEEESR GFPTQ

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