真核翻译起始因子4E结合蛋白1(EIF4EBP1)重组蛋白

真核翻译起始因子4E结合蛋白1(EIF4EBP1)重组蛋白

Recombinant Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1)

4EBP1; BP-1; PHAS-I; Phosphorylated Heat And Acid-Stable Protein Regulated By Insulin 1

[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]

Protein Protein Protein Protein Names: Eukaryotic Translation Initiation Factor 4E Binding Protein 1

Gene Names: EIF4EBP1

Size: 100µg

Source: Recombinant

Expression Expression Expression Expression Host: E.coli

Function: Function: Function: Function: Regulates eIF4E activity by preventing its assembly into the eIF4F complex.

Mediates the regulation of protein translation by hormones, growth factors and other

stimuli that signal through the MAP kinase and mTORC1 pathways.

Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Nucleus but not nucleoli, Cytoplasm

Tissue Specificity: Specificity: Specificity: Specificity: Selective cytoplasmic expression in salivary gland, pancreas, the

gastrointestinal tract and non-keratinized squamous epithelia.

[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]

Residues Residues Residues Residues: Ser2-Ile118 (Accession # Q13541), with a N-terminal His-tag.

Grade & Purity: Purity: Purity: Purity: >97%, 13.97 kDa as determined by SDS-PAGE reducing conditions.

Form & Buffer: Supplied as lyophilized form in PBS, pH 7.4.

Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg(determined by the LAL method). Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA;IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 13.97 kDa

[ PREPARATION PREPARATION PREPARATION PREPARATION ]

Reconstitute in PBS.

[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

Storage: Storage: Storage: Storage: Store at 4oC for short term storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term

storage. Avoid repeated freeze/thaw cycles.

Valid period: period: period: period: 12 months stored at -80oC.

[ BACKGROUND BACKGROUND BACKGROUND BACKGROUND]

The target protein is fused with a His-tag and its sequence is listed below. The first Met is an initiator amino acid. Moreover, Gly and Ser are added to improve the flexibility of N-terminus at both ends of the His-tag, which willincrease the chelating ability of the tag to Ni-Sepharose during purification.

MGHHHHHHSGSEF-SGGSSCSQT PSRAIPATRR VVLGDGVQLP PGDYSTTPGG TLFSTTPGGT RIIYDRKFLMECRNSPVTKT PPRDLPTIPG VTSPSSDEPP MEASQSHLRN SPEDKRAGGE ESQFEMDI

[ REFERENCES REFERENCES REFERENCES REFERENCES ]

1. Schalm S.S., et al. (2003) Curr. Biol. 13:797-806. 

2. Pause A., et al. (1994) Nature 371:762-767. 

3. Ha, Sang Hoon, et al. (2006) Cell. Signal. (England) 18 (12): 2283–2291.

4. Haghighat A., et al. (1995) EMBO J. 14:5701-5709. 5. Hara K., et al. (2002) Cell 110:177-189.

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