无翅型MMTV整合位点家族成员16(WNT16)重组蛋白

无翅型MMTV整合位点家族成员16(WNT16)重组蛋白

Recombinant Wingless Type MMTV Integration Site Family, Member 16 (WNT16)

[ PROPERTIES ]

Residues: Asn30~Lys365 (Accession # Q9UBV4),

with two N-terminal Tags, His-tag and GST-tag. Host: E. coli

Subcellular Location: Secreted, extracellular

space, extracellular matrix.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 8.6

Predicted Molecular Mass: 70.0kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, itssequence is listed below.

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYIDGDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKVDFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFKKRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPRGSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-N WMWLGIASFGVPEKLGCANL PLNSRQKELC KRKPYLLPSI REGARLGIQE CGSQFRHERW NCMITAAATTAPMGASPLFG YELSSGTKET AFIYAVMAAG LVHSVTRSCS AGNMTECSCD TTLQNGGSASEGW HWGGCSD DVQYGMWFS R KF LDFPIGNT TGKE NKVL LA MN L HNNEAGRQAVAKLMSVD CRCHGVSGSC AVKTCWKTMS SFEKIGHLLK DKYENSIQIS DKTKRKMRRREKDQRKIPIH KDDLLYVNKS PNYCVEDKKL GIPGTQGREC NRTSEGADGC NLLCCGRGYNTHVVRHVERC ECKFIWCCYV RCRRCESMTD VHTCK

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