氨基乙二酸半醛磷酸泛酰巯基乙胺基转移酶(AASDHPPT)重组蛋白

氨基乙二酸半醛磷酸泛酰巯基乙胺基转移酶(AASDHPPT)重组蛋白

Recombinant Aminoadipate Semialdehyde Phosphopantetheinyl Transferase (AASDHPPT)

AASD-PPT; CGI80; LYS2; LYS5; 4'-phosphopantetheinyl transferase; LYS5 ortholog; Alpha-aminoadipic semialdehyde dehydrogenase-phosphopantetheinyl transferase

[ PROPERTIES ]

Residues: Met1~Ser309

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q9NRN7

Host: E. coli

Subcellular Location: Cytoplasm.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 6.4

Predicted Molecular Mass: 39.5kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

MVFPAKRFCL VPSMEGVRWA FSCGTWLPSR AEWLLAVRSI QPEEKERIGQ FVFARDAKAAMAGRLMIRKL VAEKLNIPWN HIRLQRTAKG KPVLAKDSSN PYPNFNFNIS HQGDYAVLAAEPELQVGIDI MKTSFPGRGS IPEFFHIMKR KFTNKEWETI RSFKDEWTQL DMFYRNWALKESFIKAIGVG LGFELQRLEF DLSPLNLDIG QVYKETRLFL DGEEEKEWAF EESKIDEHHFVAVALRKPDG SRHQDVPSQD DSKPTQRQFT ILNFNDLMSS AVPMTPEDPS FWDCFCFTEEIPIRNGTKS

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