原钙黏素β15(PCDHb15)重组蛋白

原钙黏素β15(PCDHb15)重组蛋白

Recombinant Protocadherin Beta 15 (PCDHb15)

[ PROPERTIES ]

Residues: Val35~Leu347

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q9Y5E8

Host: E. coli

Subcellular Location: Cell membrane; Single-pass

type I membrane protein.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 4.5

Predicted Molecular Mass: 38.2kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

VMEETE RGSFVANLAN DLGLGVGELA ERGARVVSED NEQGLQLDLQ TGQLILNEKLDREKLCGPTE PCIMHFQVLL KKPLEVFRAE LLVTDINDHS PEFPEREMTL KIPETSSLGTVFPLKKARDL DVGSNNVQNY NISPNSHFHV STRTRGDGRK YPELVLDTEL DREEQAELRLTLTAVDGGSP PRSGTVQILI LVLDANDNAP EFVQALYEVQ VPENSPVGSL VVKVSARDLDTGTNGEISYS LYYSSQEIDK PFELSSLSGE IRLIKKLDFE TMSSYDLDIE ASDGGGLSGKCSVSVKVLDV NDNFPEL

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