胱天蛋白酶8(CASP8)重组蛋白

胱天蛋白酶8(CASP8)重组蛋白

Recombinant Caspase 8 (CASP8)

CAP4; FLICE; MACH; MCH5; Cysteinyl Aspartate Specific Proteinases 8; Apoptotic cysteine protease; FADD-homologous ICE/ced-3-like protease; MORT1-associated ced-3 homolog

[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Ser217~Asp384

Tags: N-terminal His-Tag

Subcellular Location: Cytoplasm. Purity: >98%

Traits: Freeze-dried powder

Buffer formulation: 10mM PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300. Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.0

Predicted Molecular Mass: 22.8kDa

Accurate Molecular Mass: 25kDa as determined by SDS-PAGE reducing conditions.

 [ USAGE ]

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do notvortex. 

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8

oC for one month. Aliquot and store at -80

oC for 12 months.

 Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37oC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date under

appropriate storage condition.

[ SEQUENCE ]

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