层粘连蛋白β2(LAMb2)重组蛋白

层粘连蛋白β2(LAMb2)重组蛋白

Recombinant Laminin Beta 2 (LAMb2)

LAM-B2; LAMS; Laminin S; Laminin B1s chain; S-laminin subunit beta

[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Gln1176~Asn1309

Tags: N-terminal His-Tag

Tissue Specificity: Lung, Heart, Brain. Subcellular Location: Secreted, extracellular space, extracellular matrix, basement membrane. Purity: >92%

Traits: Freeze-dried powder

Buffer formulation: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01%

sarcosyl and Proclin300. Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification;

Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.0

Predicted Molecular Mass: 16.1kDa

Accurate Molecular Mass: 12kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]

Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8

oC for one month. Aliquot and store at -80

oC for 12 months. 

Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37oC for 48h, and no obvious degradation and precipitation wereobserved.The loss rate is less than 5% within the expiration date under

appropriate storage condition.

[ SEQUENCE ]

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