ATP结合盒转运蛋白C8(ABCC8)重组蛋白
Recombinant ATP Binding Cassette Transporter C8 (ABCC8)
ABC-C8; PHHI; MRP8; ABC36; HHF1; HI; HRINS; SUR; SUR1; TNDM2; Sulfonylurea Receptor(Hyperinsulinemia)
[ PROPERTIES ]
Residues: Val679~Thr929
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q09428
Host: E. coli
Subcellular Location: Membrane; Multi-pass
membrane protein.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 5.7
Predicted Molecular Mass: 31.9kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
VQ IMGGYFTWTP DGIPTLSNIT IRIPRGQLTM IVGQVGCGKS SLLLAALGEM QKVSGAVFWSSLPDSEIGED PSPERETATD LDIRKRGPVA YASQKPWLLN ATVEENIIFE SPFNKQRYKMVIEACSLQPD IDILPHGDQT QIGERGINLS GGQRQRISVA RALYQHANVV FLDDPFSALD
IHLSDHLMQA GILELLRDDK RTVVLVTHKL QYLPHADWII AMKDGTIQRE GTLKDFQRSECQLFEHWKT
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