杀伤细胞免疫球蛋白样受体2DL3(KIR2DL3)重组蛋白

杀伤细胞免疫球蛋白样受体2DL3(KIR2DL3)重组蛋白

Recombinant Killer Cell Immunoglobulin Like Receptor 2DL3 (KIR2DL3)

CD158B2; Cl-6; Nkat2; Nkat2a; Nkat2b; p58; CD158 antigen-like family member B2; MHC class I NK cell receptor; Natural killer-associated transcript 2

[ PROPERTIES ]

Residues: His22~Pro341 (Accession # P43628),

with two N-terminal Tags, His-tag and GST-tag. Host: E. coli

Subcellular Location: Cell membrane;

Single-pass type I membrane protein.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 0.01%

sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 6.2

Predicted Molecular Mass: 67.3kDa

Accurate Molecular Mass: 60kDa as determined by SDS-PAGE reducing conditions.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to

give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, itssequence is listed below.

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYIDGDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKVDFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFKKRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPRGSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS- HEGVHRKPS LLAHPGPLVKSEETVILQCW SDVRFQHFLL HREGKFKDTL HLIGEHHDGV SKANFSIGPM MQDLAGTYRCYGSVTHSPYQ LSAPSDPLDI VITGLYEKPS LSAQPGPTVL AGESVTLSCS SRSSYDMYHLSREGE A HE R R FSA GPK V N G T FQA DF P L GPA THG G TY R CF G SF R DSP YEWSNSSDPLLVSV TGNPSNSWPS PTEPSSETGN PRHLHVLIGT SVVIILFILL LFFLLHRWCCNKKNAVVMDQ EPAGNRTVNR EDSDEQDPQE VTYAQLNHCV FTQRKITRPS QRPKTPPTDIIVYTELPNAE P

[ REFERENCES ]

1. Colonna M., Samaridis J. (1995) Science 268:405-408. 

2. Maenaka K., et al. (1999) Structure 7:391-398. 

3. Yu M.-C., et al. (2008) Nat. Immunol. 9:898-907. 

4. Wilson M.J., et al. (1997) Tissue Antigens 49:574-579

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