畸胎瘤衍生生长因子FRL1隐藏家族1(CFC1)重组蛋白

畸胎瘤衍生生长因子FRL1隐藏家族1(CFC1)重组蛋白

Recombinant Cripto, FRL1, Cryptic Family 1 (CFC1)

CRYPTIC; HTX2; Cryptic family protein 1

[ PROPERTIES ]

Residues: Tyr26~Leu223 (Accession # P0CG37),

with two N-terminal Tags, His-tag and T7-tag. Host: E. coli

Subcellular Location: Cell membrane;

Lipid-anchor, GPI-anchor. Secreted.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative. Predicted isoelectric point: 9.0

Predicted Molecular Mass: 25.5kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF -YQREK HNGGREEVTKVATQK HR QS P LNW TSS H FGE VTGSA EGW GP EEP LP YS RAF GEGAS A RP RCCRN GG T CV LG SF CV CPA HF T GRY CE HDQ RR SE CGA LE H GA WT LRA CH L CRCIFGALHCLP LQTPDRCDPK DFLASHAHGP SAGGAPSLLL LLPCALLHRL LRPDAPAHPRSLVPSVLQRE RRPCGRPGLG HRL

[ REFERENCES ]

1. Bamford R.N., et al. (2000) Nat. Genet. 26:365-369. 

2. Watanabe K., et al. (2008) Biochim. Biophys. Acta 1778:2671-2681. 

3. Goldmuntz E., et al. (2002) Am. J. Hum. Genet. 70:776-780. 

4. Ozcelik C., et al. (2006) Pediatr Cardiol 27:695-698.

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