角膜蛋白(KERA)重组蛋白

角膜蛋白(KERA)重组蛋白

Recombinant Keratocan (KERA)

CNA2; SLRR2B; Keratocan Proteoglycan; Keratan sulfate proteoglycan keratocan

[ PROPERTIES ]

Residues: Arg21~Asn292

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: O62702

Host: E. coli

Subcellular Location: Extracellular matrix. Secreted.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 6.8

Predicted Molecular Mass: 35.0kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

RSVRQVYEAS DPEDWTMHDF DCPRECFCPP SFPTALYCEN RGLKEIPAIP SRIWYLYLENNLIETIPEKP FENATQLRWI NLNKNKITNY GIEKGALSQL KKLLFLFLED NELEEVPSPLPRSLEQLQLA RNKVSRIPQG TFSNLENLTL LDLQHNKLLD NAFQRDTFKG LKNLMQLNMAKNALRNMPPR LPANTMQVFL DNNSIEGIPE NYFNVIPKVA FLRLNHNKLS DAGLPSSGFNVSSILDLQLS HNQLTKVPKI SAHLQHLHLD HN

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