碱性鞘磷脂磷酸二酯酶(Alk-Smase)重组蛋白

碱性鞘磷脂磷酸二酯酶(Alk-Smase)重组蛋白

Recombinant Alkaline Sphingomyelinase (Alk-Smase)

ENPP7; E-NPP 7; NPP-7; Ectonucleotide Pyrophosphatase/Phosphodiesterase 7; Sphingomyelin Phosphodiesterase; Intestinal alkaline sphingomyelinase

[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Gly22~Val439

Tags: N-terminal His-Tag

Homology: Human 78%, Mouse 90%

Tissue Specificity: Gejunum

Subcellular Location: Membrane; Single-pass type I membrane protein. Purity: >95%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method). Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification;

Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.6

Predicted Molecular Mass: 51.5kDa

Accurate Molecular Mass: 51kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex. 

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8

oC for one month. Aliquot and store at -80

oC for 12 months. 

Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37oC for 48h, and no obvious degradation and precipitation wereobserved.The loss rate is less than 5% within the expiration date under

appropriate storage condition.

[ SEQUENCE ]

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