N-乙基马来酰亚胺敏感因子(NSF)重组蛋白

N-乙基马来酰亚胺敏感因子(NSF)重组蛋白

Recombinant N-Ethylmaleimide Sensitive Factor (NSF)

SKD2; N-Ethylmaleimide Sensitive Fusion Protein; Vesicle-fusing ATPase; Vesicular-fusion protein NSF

[ PROPERTIES ]

Residues: Asp590~Asp744

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: P46459

Host: E. coli

Subcellular Location: Cytoplasm.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 6.8

Predicted Molecular Mass: 21.3kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

D DAYKSQLSCV VVDDIERLLD YVPIGPRFSN LVLQALLVLL KKAPPQGRKL LIIGTTSRKDVLQEMEMLNA FSTTIHVPNI ATGEQLLEAL ELLGNFKDKE RTTIAQQVKG KKVWIGIKKLLMLIEMSLQM DPEYRVRKFL ALLREEGASP LDFD

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！