含侧支发芽因子相关EVH1域蛋白2(SPRED2)重组蛋白

含侧支发芽因子相关EVH1域蛋白2(SPRED2)重组蛋白

Recombinant Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2)

[ PROPERTIES ]

Residues: Met1~Ala418

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q7Z698

Host: E. coli

Subcellular Location: Membrane; Cytoplasm.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 6.3

Predicted Molecular Mass: 51.2kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

MT EE T HP D DD SYI V RVKAV V MT R D DS SG GW FP QEG G GIS R VGV CKVM HP EGNGRSGFLIH GERQKDKLVV LECYVRKDLV YTKANPTFHH WKVDNRKFGL TFQSPADARAFDRGVRKAIE DLIEGSTTSS STIHNEAELG DDDVFTTATD SSSNSSQKRE QPTRTISSPTSCEHRRIYTL GHLHDSYPTD HYHLDQPMPR PYRQVSFPDD DEEIVRINPR EKIWMTGYEDYRHAPVRGKY PDPSEDADSS YVRFAKGEVP KHDYNYPYVD SSDFGLGEDP KGRGGSVIKT

QPS RGKS RRR KE D GE RS RCV YC RDMF NHEE NRR GHCQDAP DSV RT CI RRVSCMW CA DSM L YH CMS DP E G D YT DP CS C D T S DE K F C L RWMA LIA L S F L A P CMCCYLPLRAC YHCGVMCRCC GGKHKAAA

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