氨基己糖苷酶Bβ(HEXβ)重组蛋白
Recombinant Hexosaminidase B Beta (HEXb
Hex-B; ENC-1AS; HCC-7; Beta-N-acetylhexosaminidase subunit beta; Cervical cancer proto-oncogene 7 protein; N-acetyl-beta-glucosaminidase subunit beta
[ DESCRIPTION ]
Protein Names: Hexosaminidase B Beta
Synonyms: HEXb
Species: Mouse
Size: 100µg
Source: Escherichia coli-derived
Subcellular Location: Lysosome.
[ PROPERTIES ]
Residues: Pro101~Ile217 (Accession # P20060), with
N-terminal His-Tag.
Grade & Purity: >95%, 17kDa as determined by
SDS-PAGE reducing conditions.
Formulation: Supplied as lyophilized form in PBS, pH
7.4, containing 5% sucrose, 0.01% sarcosyl.
Endotoxin Level: <1.0 EU per 1µg (determined by
the LAL method).
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Molecular Mass: 14.7kDa
Predicted isoelectric point: 6.2
Mouse HEXb
[ PREPARATION ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.MGHHHHHHSGSEF- PQLQKLLVSI TLESECESFP SLSSDETYSL LVQEPVAVLKANSVWGALRG LETFSQLVYQ DSFGTFTINE SSIADSPRFP HRGILIDTSR HFLPVKTILKTLDAMAFNKF NVLHWHI
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