肌球蛋白重链1(MYH1)重组蛋白

肌球蛋白重链1(MYH1)重组蛋白

Recombinant Myosin Heavy Chain 1 (MYH1)

MYHSA1; MYHa; MyHC-2X/D; Myosin-1; Myosin Heavy Chain IIx/d; Myosin Heavy Chain 1, Skeletal Muscle, Adult

[ PROPERTIES ]

Residues: Glu1585~Met1642 linked by RTKY

Tags: Two N-terminal Tags, His-tag and GST-tag

Accession: Q5SX40

Host: E. coli

Subcellular Location: Cytoplasm, myofibril.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 10mM

PBS, pH7.4, containing 1mM DTT, 5% trehalose,

0.01% sarcosyl and preservative.

Predicted isoelectric point: 7.0

Predicted Molecular Mass: 37.5kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

 Store at 2-8

oC for one month.

 Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

RTKY EKDEEI DQLKRNHIRV VESMQSTLDA EIRSRNDAIR LKKKMEGDLN EMEIQLNHSNRM

[ REFERENCES ]

1. Church D.M., et al. (2009) PLoS Biol. 7:E1000112-E1000112.

2. Huttlin E.L., et al. (2010) Cell 143:1174-1189.

3. Weiss A., et al. (1999) Proc. Natl. Acad. Sci. U.S.A. 96:2958-2963.

4. Lu B.D., et al. (1999) Dev. Biol. 216:312-326.

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