弹性蛋白酶3B(ELA3B)重组蛋白

弹性蛋白酶3B(ELA3B)重组蛋白

Recombinant Elastase 3B (ELA3B)

CELA3B; Chymotrypsin Like Elastase Family Member 3B; Protease E; Fecal Elastase; Elastase 1; Cholesterol-Binding Pancreatic Protease; Pancreatic Endopeptidase E

[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Val28~Asn269

Tags: N-terminal His-Tag

Tissue Specificity: Pancreas. Purity: >92%

Traits: Freeze-dried powder

Buffer formulation: 10mM PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300. Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.6

Predicted Molecular Mass: 27.3kDa

Accurate Molecular Mass: 27kDa as determined by SDS-PAGE reducing conditions. 

[ USAGE ]

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do notvortex.

 [ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8

oC for one month. Aliquot and store at -80

oC for 12 months. 

Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37oC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date under

appropriate storage condition.

[ SEQUENCE ]

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