成纤维细胞生长因子10(FGF10)重组蛋白

成纤维细胞生长因子10(FGF10)重组蛋白

Recombinant Fibroblast Growth Factor 10 (FGF10)

Keratinocyte growth factor 2

[ PROPERTIES ]

Residues: Gln37~Ser215

Tags: Two N-terminal Tags, His-tag and GST-tag

Accession: P70492

Host: E. coli

Subcellular Location: Secreted.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 10mM PBS,

pH7.4, containing 1mM DTT, 5% trehalose, 0.01%

sarcosyl and preservative.

Predicted isoelectric point: 9.2

Predicted Molecular Mass: 47.1kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

 Store at 2-8

oC for one month.

 Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

QALG QDMVSPEATN SSSSSSSSSS SSSFSSPSSA GRHVRSYNHL QGDVRWRKLFSFTKYFLKIE KNGKVSGTKK ENCPYSILEI TSVEIGVVAV KAINSNYYLA MNKKGKLYGSKEFNNDCKLK ERIEENGYNT YASFNW QHNG RQMYVALNGK GAPRRGQKTRRKNTSAHFLP MVVHS

[ REFERENCES ]

1. Yamasaki M., et al. (1996) J. Biol. Chem. 271:15918-15921.

2. Suzuki K., et al. (2000) FEBS Lett. 481:53-56.

3. Kelly R.G., et al. (2001) Dev. Cell 1:435-440.

4. Ishiwata T., et al. (2002) Exp. Mol. Pathol. 73:181-190.

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