延伸蛋白A(ELOA)重组蛋白

延伸蛋白A(ELOA)重组蛋白

Recombinant Elongin A (ELOA)

Elo-A; TCEB3; SIII p110; TCEB3A; Transcription Elongation Factor B Polypeptide 3; Elongin 110 kDa subunit; RNA polymerase II transcription factor SIII subunit A1

[ PROPERTIES ]

Residues: Val76~Ser345 (Accession # Q8CB77),

with two N-terminal Tags, His-tag and T7-tag. Host: E. coli

Subcellular Location: Nucleus.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 9.3

Predicted Molecular Mass: 34.6kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF - VPVER NSEAEDQDFEKNNS RK RP R D AL QREE E LEG NYQESWKP SG SRS YSP E H RQ KK HKK L SEP ERP HKVA HS HE KRDE RK R CHK VSP PY SS DPE SS DYG HV QSP PPS SP H QMYTDLSRSPEEDQ EPIISHQKPG KVHSNTFQDR LGVSHLGEQG KGAVSHHKQH RSSHKEKHPADAREDEKISA VSREKSHKAS SKEESRRLLS GDSAKEKLPS SVVKKDKDRE GSSLKKKFSPALDVASDNHF KKPKHKDSEK AKSDKNKQSV DGVDS

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