载脂蛋白A2(APOA2)重组蛋白

载脂蛋白A2(APOA2)重组蛋白

Recombinant Apolipoprotein A2 (APOA2)

Apo-A2; Apolipoprotein A-II; ProapoA-II; Truncated apolipoprotein A-II

[ PROPERTIES ]

Residues: Gln24~Gln100 (Accession # P02652),

with two N-terminal Tags, His-tag and GST-tag. Host: E. coli

Subcellular Location: Secreted. Cell membrane;

Single-pass type I membrane protein.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 5.9

Predicted Molecular Mass: 40.7kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, itssequence is listed below.

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYIDGDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKVDFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFKKRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPRGSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- QAKEPCV ESLVSQYFQTVTDYGKDLME KVKSPELQAE AKSYFEKSKE QLTPLIKKAG TELVNFLSYF VELGTQPATQ

[ REFERENCES ]

1. Knott T.J., et al. (1984) Biochem. Biophys. Res. Commun. 120:734-740. 

2. Moore M.N., et al. (1984) Biochem. Biophys. Res. Commun. 123:1-7. 

3. Sharpe C.R., et al. (1984) Nucleic Acids Res. 12:3917-3932. 

4. Lackner K.J., et al. (1985) Nucleic Acids Res. 13:4597-4608

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