糖原磷酸化酶L(PYGL)重组蛋白

糖原磷酸化酶L(PYGL)重组蛋白

Recombinant Glycogen Phosphorylase, Liver (PYGL)

GPLL; GPBB; Hers Disease; Glycogen Storage Disease Type VI

[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]

Protein Protein Protein Protein Names: Glycogen Phosphorylase, Liver

Synonyms Synonyms Synonyms Synonyms: PYGL, GPLL

Species: Species: Species: Species: Human

Size: 100µg

Source: Escherichia coli-derived

[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]

Residues Residues Residues Residues: Asn633~Glu820 (Accession # P06737), with N-terminal His-Tag.

Grade & Purity: Purity: Purity: Purity: >97%, 22.6 kDa as determined by SDS-PAGE reducing conditions.

Formulation Formulation Formulation Formulation: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.

Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method). Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 22.6 kDa

Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 5.2

[ PREPARATION PREPARATION PREPARATION PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.

Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months . Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was

determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obviousdegradation and precipitation were observed. (referring from China Biological Products Standard, which wascalculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date underappropriate storage condition.

[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES]

The target protein is fused with one N-terminal His-tag, its sequence is listed below.

NDPMVGSK LKVIFLENYR VSLAEKVIPA TDLSEQISTA GTEASGTGNM KFMLNGALTI GTMDGANVEMAEEAGEENLF IFGMRIDDVAALDKKGYEAK EYYEALPELK LVIDQIDNGF FSPKQPDLFK DIINMLFYHDRFKVFADYEA YVKCQDKVSQ LYMNPKAWNT MVLKNIAASG KFSSDRTIKE

[ REFERENCES REFERENCES REFERENCES REFERENCES ]

1. Livanova NB., et al. (2002) Biochemistry (Moscow) 67 (10): 1089–1998.

 2. Palm D., et al. (1990) Biochemistry 29 (5): 1099–1107.

3. Browner MF., et al. (2005) Trends in Biochemical Science 17 (2): 66–71.

4. Johnson LN., et al. (1992) FASEB Journal 6 (6): 2274–82. 5. Newgard CB., et al. (1989) Critical Reviews Biochemistry and Molecular Biology 24 (1): 69–99.

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