胰岛细胞自身抗原1(ICA1)重组蛋白

胰岛细胞自身抗原1(ICA1)重组蛋白

Recombinant Islet Cell Autoantigen 1 (ICA1)

ICA69; ICAp69; ICA-p69; 69 kDa islet cell autoantigen

[ PROPERTIES ]

Residues: Met1~Gly257 (Accession # P97411),

with two N-terminal Tags, His-tag and S-tag. Host: E. coli

Subcellular Location: Cytoplasm. Golgi

apparatus membrane; Peripheral membrane

protein. Cytoplasmic vesicle, secretory vesicle

membrane; synaptic vesicle membrane. Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 8.8

Predicted Molecular Mass: 35.9kDa

Accurate Molecular Mass: 26&32&37kDa as determined by SDS-PAGE reducing

conditions.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to

give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and S-tag, itssequence is listed below.

MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF -MS GHK CYSWE LQ D RFA QDKS VV NKMQ QKYW ETKQAFIKAT GK KE DE HVVASDADLDAKLE LFHSIQRTCL DLSKAIVLYQ KRICFLSQEE NELGKFLRSQ GFQDKTRAGKMMQATGKALC FSSQQRLALR NPLCRFHQEV ETFRHRAISD TWLTVNRMEQ YRTEYRGALLWMK DV SQE L D PD LYK QMEK F RK VQ T QV R LA KK NF DK LKM D VC QKV D L LGASRCNLLSHML ATYQTTLLHF WEKTSHTMAA IHESFKG

[ REFERENCES ]

1. Gaedigk R., et al. (1996) Genomics 38:382-391.

 2. Karges W.J.P., et al. (1997) Biochim. Biophys. Acta 1360:97-101.

 3. Church D.M., et al. (2009) PLoS Biol. 7:E1000112-E1000112.

 4. Pilon M., et al. (2000) Mol. Biol. Cell 11:3277-3288.

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