平足蛋白(PDPN)重组蛋白

平足蛋白(PDPN)重组蛋白

Recombinant Podoplanin (PDPN)

GP40; GP36; Gp38; HT1A-1; OTS8; PA2.26; T1A; T1A-2; Aggrus; Glycoprotein 36; PA2.26 antigen; T1-alpha; Lung Type I Cell Membrane Associated Glycoprotein

[ PROPERTIES ]

Residues: Ala23~Leu143 linked with

RKWMETHRQQ LRKMVCQQ

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q86YL7

Host: E. coli

Subcellular Location: Membrane; Single-pass type

I membrane protein. Cell projection, filopodium

membrane. Lamellipodium membrane. Microvillus

membrane. Ruffle membrane.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 7.9

Predicted Molecular Mass: 18.8kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

 Store at 2-8

oC for one month.

 Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

ARAPSLSG APAPTPPAPP PSRSSRLGLW PRCFLIFPQL RILLLGPQES NNSTGTMWKVSALLFVLGSA SLWVLAEGAS TGQPEDDTET TGLEGGVAMP GAEDDVVTPG TSEDRYKSGLTTL RKWMETHRQQ LRKMVCQQ

[ REFERENCES ]

1. Ma T., et al. (1998) Am. J. Respir. Cell Mol. Biol. 19:143-149.

2. Zimmer G., et al. (1999) Biochem. J. 341:277-284.

3. Martin-Villar E., et al. (2005) Int. J. Cancer 113:899-910.

4. Kato Y., et al. (2003) J. Biol. Chem. 278:51599-51605.

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