乳酸脱氢酶A(LDHA)重组蛋白

乳酸脱氢酶A(LDHA)重组蛋白

Recombinant Lactate Dehydrogenase A (LDHA)

LDH-A; LDH-M; LDH1; PIG19; Cell proliferation-inducing gene 19 protein; LDH muscle subunit; Renal carcinoma antigen NY-REN-59

[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]

Protein Protein Protein Protein Names: Lactate Dehydrogenase A

Gene Names: LDHA, Ldh-1, Ldh1

Size: 100µg

Source: Recombinant

Expression Expression Expression Expression Host: E.coli

Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cytoplasm.

[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]

Residues Residues Residues Residues: Met1~Phe332 (Accession # P04642), with a N-terminal His-tag.

Grade & Purity: Purity: Purity: Purity: >97%, 38 kDa as determined by SDS-PAGE reducing conditions.

Form & Buffer: Supplied as lyophilized form in PBS, pH 7.4.

Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method). Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 38 kDa

[ PREPARATION PREPARATION PREPARATION PREPARATION ]

Reconstitute in PBS.

[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

Storage: Storage: Storage: Storage: Store at 4oC for short time storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term storage.

Avoid repeated freeze/thaw cycles.

Valid period: period: period: period: 12 months stored at -80oC.

[ BACKGROUND BACKGROUND BACKGROUND BACKGROUND]

The target protein is fused with a His-tag and its sequence is listed below. The first Met is an initiator amino acid. Moreover, Gly and Ser are added to improve the flexibility of N-terminus at both ends of the His-tag, which willincrease the chelating ability of the tag to Ni-Sepharose during purification.

MGHHHHHHSGSEF-MAALKDQLIV NLLKEEQVPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKGEMMDLQHGSL FLKTPKIVSS KDYSVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPQCKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPVWSGVNVAGVS LKSLNPQLGT DADKEQWKDV HKQVVDSAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRVHPISTMIKGL YGIKEDVFLS VPCILGQNGI SDVVKVTLTP DEEARLKKSA DTLWGIQKEL QF

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