蛋白激酶D1(PKD1)重组蛋白

蛋白激酶D1(PKD1)重组蛋白

Recombinant Protein Kinase D1 (PKD1)

PRKD1; PRK-D1; PKC-MU; PKCM; PK-D1; PRKCM; Protein Kinase C,Mu

[ PROPERTIES ]

Residues: Glu349~Gly612 (Accession # Q62101),

with two N-terminal Tags, His-tag and T7-tag. Host: E. coli

Subcellular Location: Cytoplasm . Cell

membrane . Golgi apparatus, trans-Golgi

network.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 5.7

Predicted Molecular Mass: 32.7kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSG LVPRGS H MASMTGGQQM GRGSEF - EG SDDNDSERNSGLM DDM DEAM VQD TEMA LA E GQSG GA EMQD PDA D QE DS N R TISPS TS N NIPLM RV V QS V K HTK R RS S T VM KE GWM V HY T S KD T L RK R HYW RL DS K CI T L FQNDTGSRYYK EIPLSEILCL EPAKPSALTP VGATPHCFEI TTANVVYYVG ENVVNPSSSPPNNSVLPSGI GPDVARMWEV AIQHALMPVI PKGSSVGSGS NSHKDISVSI SVSNCQIQENVDISTVYQIF PDEVLGSGQF GIVYGGKHRK TG

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