血管内皮生长因子A(VEGFA)重组蛋白

血管内皮生长因子A(VEGFA)重组蛋白

Recombinant Vascular Endothelial Growth Factor A (VEGFA)

VEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor

[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Ala27~Arg146

Tags: N-terminal His-Tag

Tissue Specificity: Spleen. Subcellular Location: Secreted. Cell membrane; Peripheral membrane protein. Purity: >98%

Traits: Freeze-dried powder

Buffer formulation: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01%

sarcosyl and Proclin300. Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive

Labeling. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.5

Predicted Molecular Mass: 17.8kDa

Accurate Molecular Mass: 18kDa as determined by SDS-PAGE reducing conditions. 

[ USAGE ]

Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8

oC for one month. Aliquot and store at -80

oC for 12 months. 

Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37oC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date under

appropriate storage condition.

[ SEQUENCE ]

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