Toll样受体5(TLR5)重组蛋白

Toll样受体5(TLR5)重组蛋白

Recombinant Toll Like Receptor 5 (TLR5)

CD285; SLEB1; TIL3; Toll/Interleukin-1 Receptor-Like Protein 3

[ PROPERTIES ]

Residues: Asn46~Ala205

Tags: Two N-terminal Tags, His-tag and S-tag

Accession: O60602

Host: E. coli

Subcellular Location: Membrane; Single-pass

type I membrane protein.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 10mM

PBS, pH7.4, containing 1mM DTT, 5% trehalose,

0.01% sarcosyl and preservative.

Predicted isoelectric point: 6.4

Predicted Molecular Mass: 23.8kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

NTTER LLLSFNYIRT VTASSFPFLE QLQLLELGSQ YTPLTIDKEA FRNLPNLRIL DLGSSKIYFLHPDAFQGLFH LFELRLYFCG LSDAVLKDGY FRNLKALTRL DLSKNQIRSL YLHPSFGKLNSLKSIDFSSN QIFLVCEHEL EPLQGKTLSF FSLAA

[ REFERENCES ]

1. Chaudhary P.M., et al. (1998) Blood 91:4020-4027. 

2. Nakajima T., et al. (2008) Immunogenetics 60:727-735. 

3. Wlasiuk G., et al. (2009) Mol. Biol. Evol. 26:937-949.

 4. Gregory S.G., et al. (2006) Nature 441:315-321.

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