淋巴毒素β(LTb)重组蛋白

淋巴毒素β(LTb)重组蛋白

Recombinant Lymphotoxin Beta (LTb)

LT-B; p33; TNFC; TNF-C; TNFSF3; Tumor necrosis factor ligand superfamily member 3; Tumor Necrosis Factor C

[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Ile58~Met304

Tags: N-terminal His-Tag

Tissue Specificity: Spleen. Subcellular Location: Membrane; Single-pass type II membrane protein. Purity: >98%

Traits: Freeze-dried powder

Buffer formulation: 10mM PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300. Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.8

Predicted Molecular Mass: 27.6kDa

Accurate Molecular Mass: 32kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are as

follows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to

give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. 

[ USAGE ]

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do notvortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8

oC for one month. Aliquot and store at -80

oC for 12 months. 

Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37oC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date under

appropriate storage condition. 

[ SEQUENCE ]

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