规格:
50µg/200µg/500µg/1mg/5mg
货号:
IC823Hu
外核苷酸焦磷酸酶/磷酸二酯酶2(ENPP2)重组蛋白
Recombinant Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2)
ATX; ATX-X; LysoPLD; NPP2; PD-IALPHA; PDNP2; Autotaxin; Extracellular lysophospholipase D
[ PROPERTIES ]
Residues: Arg637~Lys905 (Accession # Q13822),
with two N-terminal Tags, His-tag and T7-tag. Host: E. coli
Subcellular Location: Secreted.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 6.5
Predicted Molecular Mass: 35.0kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.
MGSSHHHHHH SSG LVPRGSH MASMTGGQQM GRGSEF -RLHT KGSTEERH LLYGRPAVLYRT RYDILYHTDF ESGYSEIFLM PLWTSYTVSK QAEVSSVPDH LTSCVRPDVRVSPSFSQNCL AYKNDKQMSY GFLFPPYLSS SPEAKYDAFL VTNMVPMYPA FKRVWNYFQRVLVKKYASER NGVNVISGPI FDYDYDGLHD TEDKIKQYVE GSSIPVPTHY YSIITSCLDFTQPADKCDGP LSVSSFILPH RPDNEESCNS SEDESKWVEE LMKMHTARVR DIEHLTSLDFFRKTSRSYPE ILTLK
[ REFERENCES ]
1. Murata J., et al. (1994) J. Biol. Chem. 269:30479-30484.
2. Kawagoe H., et al. (1995) Genomics 30:380-384.
3. Lee H.Y., et al. (1996) Biochem. Biophys. Res. Commun. 218:714-719.
4. Giganti A., et al. (2008) J. Biol. Chem. 283:7776-7789.
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