尿苷胞苷激酶2(UCK2)重组蛋白
Recombinant Uridine Cytidine Kinase 2 (UCK2)
UK; UMPK; Uridine Monophosphate Kinase; Cytidine monophosphokinase 2; Testis-specific protein TSA903; Uridine monophosphokinase 2
[ PROPERTIES ]
Residues: Gly18~His261
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q9BZX2
Host: E. coli
Subcellular Location: Cytosol.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 7.1
Predicted Molecular Mass: 31.1kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
GEP FLIGVSGGTA SGKSSVCAKI VQLLGQNEVD YRQKQVVILS QDSFYRVLTSEQKAKALKGQ FNFDHPDAFD NELILKTLKE ITEGKTVQIP VYDFVSHSRK EETVTVYPADVVLFEGILAF YSQEVRDLFQ MKLFVDTDAD TRLSRRVLRD ISERGRDLEQ ILSQYITFVKPAFEEFCLPT KKYADVIIPR GADNLVAINL IVQHIQDILN GGPSKRQTNG CLNGYTPSRKRQASESSSRP H
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