羟酸氧化酶1(HAO1)重组蛋白

羟酸氧化酶1(HAO1)重组蛋白

Recombinant Hydroxyacid Oxidase 1 (HAO1)

GOX; Glycolate Oxidase

[ PROPERTIES ]

Residues: Ser113~Lys369

Tags: Two N-terminal Tags, His-tag and GST-tag

Accession: Q9WU19

Host: E. coli

Subcellular Location: Peroxisome.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 6.8

Predicted Molecular Mass: 60.5kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

 Store at 2-8

oC for one month.

 Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

SSIEEVAE AGPEALRWMQ LYIYKDREIS RQIVKRAEKQ GYKAIFVTVD TPYLGNRIDDVRNRFKLPPQ LRMKNFETND LAFSPKGNFG DNSGLAEYVA QAIDPSLSWD DITWLRRLTSLPIVVKGILR GDDAKEAVKH GVDGILVSNH GARQLDGVPA TIDVLPEIVE AVEGKVEVFLDGGVRKGTDV LKALALGAKA VFVGRPIIWG LAFQGEKGVQ DVLEILKEEF RLAMALSGCQNVKVIDKTLV RKNPLAVSK

[ REFERENCES ]

1. Kohler S.A., et al. (1999) J. Biol. Chem. 274:2401-2407.

2. Williams E.L., et al. (2000) Biochim. Biophys. Acta 1493:246-248.

3. Spence S.E., et al. (1992) Genomics 12:403-404.

4. Holmes R.S. (1976) Biochem. Genet. 14:981-987.

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