神经蛋白聚糖(NCAN)重组蛋白

神经蛋白聚糖(NCAN)重组蛋白

Recombinant Neurocan (NCAN)

CSPG3; NEUR; Neurocan Core Protein; Chondroitin Sulfate Proteoglycan 3; Neurocan Proteoglycan

[ PROPERTIES ]

Residues: His1008~Lys1273

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: O14594

Host: E. coli

Subcellular Location: Secreted.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 5.6

Predicted Molecular Mass: 33.6kDa

Accurate Molecular Mass: 38kDa as determined by SDS-PAGE reducing conditions.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to

give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

HSD PCENNPCLHG GTCNANGTMY GCSCDQGFAG ENCEIDIDDC LCSPCENGGTCI DE V NG FV C LC LPSYG GS F CEK D TEG CDR GW HKF QG HCY RY FA HRRAWEDAEKDCRRRS GHLTSVHSPE EHSFINSFGH ENTWIGLNDR IVERDFQWTD NTGLQFENWRENQP DN F FAG GE D CVVMVA H ES GRW N DVP C NY N LPY V CK K GTV L C GP PPAVENASLIGAR KAKYNVHATV RYQCNEGFAQ HHVATIRCRS NGKWDRPQIV CTK

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