对氧磷酶1(PON1)重组蛋白

对氧磷酶1(PON1)重组蛋白

Recombinant Paraoxonase 1 (PON1)

ESA; PON; Esterase A; Serum paraoxonase/arylesterase 1; Aromatic esterase 1; A-esterase 1; Serum aryldialkylphosphatase 1

[ PROPERTIES ]

Residues: Ala187~Asp354 (Accession # P52430),

with N-terminal His-Tag.

Host: E. coli

Subcellular Location: Secreted, extracellular

space. Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 1mM DTT, 5% trehalose, 0.05%

sarcosyl and preservative.

Predicted isoelectric point: 5.7

Predicted Molecular Mass: 20.3kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGSEF- ADPY LRSWEMYLGL SWSNVVYYSP DKVQVVAEGF DFANGIGISLDGKYVYIAEL LAHKIHVYEK HANWTLTPLK VLNFDTLVDN ISVDPVTGDL WVGCHPNGMRIFFYDAENPP GSEVLRIQNI LSEDPKITVV YAENGTVLQG TTVASVYKGK LLIGTVFHKA LYCD

[ REFERENCES ]

1. Sorenson R.C., et al. (1995) Proc. Natl. Acad. Sci. U.S.A. 92:7187-7191. 

2. Shih D.M., et al. (1996) J. Clin. Invest. 97:1630-1639. 

3. Li W.F., et al. (1997) Pharmacogenetics 7:137-144. 

4. Yang F., et al. (2005) FEBS Lett. 579:3713-3717.

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