冠蛋白1A(CORO1A)重组蛋白

冠蛋白1A(CORO1A)重组蛋白

Recombinant Coronin 1A (CORO1A)

CLABP; p57; CLIPINA; HCORO1; TACO; Coronin-1; Coronin,Actin-Binding Protein,1A; Coronin-like protein A; Coronin-like protein p57; Tryptophan aspartate-containing coat protein

[ PROPERTIES ]

Residues: Met1~Glu204

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q91ZN1

Host: E. coli

Subcellular Location: Cytoplasm. Cytoplasmic

vesicle. Cytoskeleton. Membrane.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 5.8

Predicted Molecular Mass: 25.7kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

MS R QV V RS S K FR HV F G QPA K AD Q CY E DV RV SQ T TW D SG F C AV NP K FM A LICEASGGGAFL VLPLGKTGRV DKNVPLVCGH TAPVLDIAWC PHNDNVIASG SEDCTVMVWEIPDGGLVLPL REPVVTLEGH TKRVGIVAWH PTAQNVLLSA GCDNVILVWD VGTGAAVLTLGPDVHPDTIY SVDWSRDGAL ICTSCRDKRV RVIEMSRQVVRSSK FRHVFGQPAKADQCYEDVRV SQTTWDSGFC AVNPKFMALI CEASGGGAFL VLPLGKTGRV DKNVPLVCGHTAPVLDIAWC PHNDNVIASG SEDCTVMVWE IPDGGLVLPL REPVVTLEGH TKRVGIVAWHPTAQNVLLSA GCDNVILVWD VGTGAAVLTL GPDVHPDTIY SVDWSRDGAL ICTSCRDKRVRVIE

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