α-胞衬蛋白(SPTAN1)重组蛋白

α-胞衬蛋白(SPTAN1)重组蛋白

Recombinant Alpha-Fodrin (SPTAN1)

SPTAN1; NEAS; (ALPHA)II-SPECTRIN; Spectrin Alpha Non-Erythrocytic 1; Spectrin Alpha Chain,Brain; Spectrin, non-erythroid alpha subunit

[ PROPERTIES ]

Residues: Trp1004~Arg1181 (Accession # P16086),

with N-terminal His-Tag.

Host: E. coli

Subcellular Location: Cytoplasm, cytoskeleton,

cell cortex. Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 5.3

Predicted Molecular Mass: 22.0kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGSEF - WWKVEVN DRQGFVPAAY VKK LDPAQSA SRE NLLEEQG

SIALRQGQID NQTRITKEAG SVSLRMKQVE ELYQSLLELG EKRKGMLEKS CKKFMLFREA

NELQQWINEK EAALTSEEVG ADLEQVEVLQ KKFDDFQKDL KANESRLKDI NKVAEDLESE

GLMAEEVQAV QQQEVYGMMP R

[ REFERENCES ]

1. Hong W., Doyle D. (1989) J. Biol. Chem. 264:12758-12764. 2. Nicolas G., et al. (2002) Mol. Cell. Biol. 22:3527-3536. 3. Lehtonen S., et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102:9814-9819. 4. Hoffert J.D., et al. (2006) Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164.

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