信号素5B(SEMA5B)重组蛋白

信号素5B(SEMA5B)重组蛋白

Recombinant Semaphorin 5B (SEMA5B)

SEMAG; SemG; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 5B

[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Leu36~Glu161

Tags: N-terminal His-Tag

Tissue Specificity: Brain, Ovary, Lung. Subcellular Location: Membrane; Single-pass type III membrane protein. Purity: >95%

Traits: Freeze-dried powder

Buffer formulation: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01%

sarcosyl and Proclin300. Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification;

Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.0

Predicted Molecular Mass: 14.9kDa

Accurate Molecular Mass: 14kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]

Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8

oC for one month. Aliquot and store at -80

oC for 12 months.

 Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37oC for 48h, and no obvious degradation and precipitation wereobserved.The loss rate is less than 5% within the expiration date under

appropriate storage condition. 

[ SEQUENCE ]

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